Human brain microvascular endothelial cell pairs model tissue-level blood–brain barrier function
نویسندگان
چکیده
منابع مشابه
Tissue plasminogen activator expression and barrier properties of human brain microvascular endothelial cells.
BACKGROUND Tissue plasminogen activator (tPA) regulates fibrinolysis and is routinely used as ischemic stroke pharmacotherapy. We hypothesized that brain microvascular tPA expression and barrier properties of endothelial cells are substantially related. METHODS Human brain microvascular endothelial cells were incubated with two agents known to modify cAMP pathways: forskolin and rolipram. We ...
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Aflatoxins are mycotoxins produced by Aspergillus spp. Although AFB1 is implicated as a carcinogen in hepatocellular carcinoma, brain autopsies in affected areas have revealed its presence in 81% of cases. Given its haematogenous spread, here we determined the cytotoxic effects of AFB1 on primary human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, huma...
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The invasive ability of the blood-borne fungal pathogen Cryptococcus neoformans can be enhanced through interactions with host plasma components, such as plasminogen. Previously we showed by in vitro studies that plasminogen coats the surface of C. neoformans and is converted to the active serine protease, plasmin, by host plasminogen activators. Viable, but not formaldehyde- or sodium azide-ki...
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Epithelial and endothelial cells (EC) are building paracellular barriers which protect the tissue from the external and internal environment. The blood-brain barrier (BBB) consisting of EC, astrocyte end-feet, pericytes and the basal membrane is responsible for the protection and homeostasis of the brain parenchyma. In vitro BBB models are common tools to study the structure and function of the...
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Background Atrial natriuretic peptide (ANP) regulates blood pressure and volume (for review see [1]). Its receptor, the guanylyl cyclase-A (GC-A) is expressed in vascular endothelium and mediates increases in intracellular cyclic GMP levels, but the functional relevance is controversial. Notably, mice with endothelial-restricted GC-A deletion (EC GC-A KO mice) exhibit significant hypervolemia a...
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ژورنال
عنوان ژورنال: Integrative Biology
سال: 2020
ISSN: 1757-9708
DOI: 10.1093/intbio/zyaa005